Identification of candidate antimicrobial peptides derived from abalone hemocyanin

分类： 生物学 >> 生物物理学 >> 免疫学 提交时间： 2016-05-11

Zhuang, Jun Wu, Zujian Xie, Lianhui Zhuang, Jun Wu, Zujian Xie, Lianhui Coates, Christopher J. Zhu, Hongtao Zhu, Ping Zhu, Hongtao

摘要： Hemocyanins present in invertebrate hemolymph are multifunctional proteins, responsible for oxygen transport and contributing to innate immunity through phenoloxidase-like activity. In arthropods, hemocyanin has been identified as a source of broad-spectrum antimicrobial peptides during infection. Conversely, no hemocyanin-derived antimicrobial peptides have been reported for molluscs. The present study describes a putative antimicrobial region, termed haliotisin, located within the linking sequence between the alpha-helical domain and beta-sheet domain of abalone (Haliotis tuberculata) hemocyanin functional unit E. A series of synthetic peptides based on overlapping fragments of the haliotisin region were tested for their bactericidal potential. Incubating Gram-positive and Gram-negative bacteria in the presence of certain haliotisin peptides, notably peptides 3-4-5 (DTFDYKKFGYRYDSLELEGRS ISRIDELIQQRQEKDRTFAGFLLKGFGTSAS) led to reductions in microbial growth. Furthermore, transmission electron micrographs of haliotisin-treated bacteria revealed damages to the microbial cell wall. Data discussed here provides the first evidence to suggest that molluscan hemocyanin may act as a source of anti-infective peptides. (C) 2014 Elsevier Ltd. All rights reserved.

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